This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates gradient separation of nucleic acid bases.
Compounds that are used in this study includes:
2. Uracil
3. 5-Fluorocytosine
4. Cytosine
Figure 1: Structure of the compounds analysed.
|
Column: |
ACQUITY UPLC BEH HILIC, 2.1 x 100 mm, 1.7 μm |
|
Part Number: |
186003461 |
|
Mobile Phase A: |
20 mM CH3COONH4, 0.05% CH3COOH in 50:40:10 ACN:MeOH:H2O |
|
Mobile Phase B: |
4 mM CH3COONH4, 0.01% CH3COOH in 95:3:2 ACN:MeOH:H2O |
|
Flow Rate: |
0.790 mL/min |
|
Injection Volume: |
0.8 μL |
|
Sample Concentration: |
25 μg/mL |
|
Sample Diluent: |
75:25 ACN:MeOH with 0.2% HCOOH |
|
Temperature: |
30 °C |
|
Detection: |
UV @ 254 nm |
|
Sampling Rate: |
20 pts/sec |
|
Time Constant: |
0.1 |
|
Instrument: |
Waters ACQUITY UPLC with ACQUITY TUV |
|
Time(min) |
Profile |
|
|---|---|---|
|
%A |
%B |
|
|
0.0 |
0.1 |
99.9 |
|
0.37 |
0.1 |
99.9 |
|
1.71 |
99.9 |
0.1 |
|
1.74 |
0.1 |
99.9 |
|
1.98 |
0.1 |
99.9 |
Figure 2: Chromatogram of 1. 5-Fluorouracil 2. Uracil 3. 5- Fluorocytosine 4. Cytosine
WA60138, August 2009
