• Application Note

Analysis of Stevia Related Compounds Using ACQUITY UPLC BEH Amide Columns

Analysis of Stevia Related Compounds Using ACQUITY UPLC BEH Amide Columns
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of stevia related compounds using ACQUITY UPLC BEH Amide Columns.

Introduction

The compounds used in this study are:

  1. Steviol
  2. Stevioside
  3. Rebaudioside A
  4. Rebaudioside C

Experimental

Chromatographic Conditions

Column:

ACQUITY UPLC BEH Amide 2.1 x 100 mm, 1.7 µm

Part Number:

186004801

Mobile Phase A:

80/20 MeCN/H2O with 0.2% triethylamine [TEA]

Mobile Phase B:

30/70 MeCN/H2O with 0.2% triethylamine [TEA]

Flow Rate:

0.20 mL/min

Injection Volume:

1.3 μL (PLNO)

Sample Concentration:

5 mg/mL

Sample Diluent:

50/50 MeCN/H2O

Column Temperature:

35 °C

Strong Needle Wash:

20/80 MeCN/H2O (800 μL)

Weak Needle Wash:

75/25 MeCN/H2O (500 μL)

Seal Wash:

50/50 MeCN/H2O

Instrument:

Waters ACQUITY UPLC with ELSD

ELSD Conditions

Gain:

200

Pressure:

40 psi

Drift Tube Temperature:

40 °C

Nebulizer:

Cooling

Data Rate:

10 pps

Filter Time Constant:

Normal

Results and Discussion

Results

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