• Application Note

Analysis of β-Blockers on Atlantis T3 Columns

Analysis of β-Blockers on Atlantis T3 Columns
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of β-blockers on Atlantis T3 Columns.

Introduction

The compounds used in this study are - 

  1. Atenolol
  2. Pindolol
  3. Metoprolol
  4. Oxprenolol
  5. Labetalol
  6. Propranolol

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 150 mm, 5 μm

Part number:

186003747

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM HCOONH4, pH 3.0

Flow rate:

2.0 mL/min

Injection volume:

10 μL

Sample:

Atenolol (40μg/mL),

Pindolol (20 μg/mL),

Metoprolol (60 μg/mL),

Oxprenolol (40 μg/mL),

Labetalol (60 μg/mL),

Propranolol (20 μg/mL)

in H2O/ACN (95/5)

Column temperature:

30 °C

Sample temperature:

15 °C

Detection:

UV @ 280 nm

Sampling rate:

5 point/sec

Filter response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Gradient

Time (min)

Profile

%A

%B

%C

0.00

85

5

10

3.00

65

25

10

10.00

65

25

10

11.00

85

5

10

15.00

85

5

10

Results and Discussion

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WA60223, January 2009

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