Aflatoxins in Red Pepper

In this application brief, we determine aflatoxins in red pepper.

Aflatoxins are naturally occurring mycotoxins. Aflatoxins often cause disease even when ingested in minute amounts and are most commonly known for causing acute, or chronic liver disease, and liver cancer.

Pretreatment

1. Weigh 50 g ground sample with 5 g sodium chloride and place in blender jar.

2. Add 100 mL 80:20 methanol: water (v/v) to jar.

3. Blend at high speed for 1 minute.

4. Filter extract with fluted filter paper. Collect filtrate in a clean vessel.

5. Pipette or pour 65 mL filtered extract into a clean vessel.

6. Dilute extract with 60 mL of phosphate buffer saline. Mix well.

7. Filter extract through glass microfiber filter into a clean vessel.