This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of pheniramine using XTerra RP18 columns.
Pheniramine has been analyzed in this study.
            
        | 
                     Column:  | 
                     XTerra RP18 4.6 x 150 mm, 5 μm (p/n: 186000492)  | 
            
| 
                     Mobile phase:  | 
                     At pH 3.0: H2O/ACN/100 mM, NH4COOH, pH 3.0, 55:35:10 At pH 7.0: H2O/ACN/100 mM, NH4HCO3, pH 7.0, 30:60:10 At pH 10.0: H2O/ACN/100 mM, NH4HCO3, pH 10.0, 60:30:10  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     5 μL of 250 μg/mL  | 
            
| 
                     Temperature:  | 
                     30 °C  | 
            
| 
                     Detection:  | 
                     UV @ 265 nm  | 
            
| 
                     Instrument:  | 
                     Alliance 2695, 2996 PDA  | 
            
| 
   Mobile phase pH  | 
  
   USP Tailing  | 
 
| 
   3.0  | 
  
   1.19  | 
 
| 
   7.0  | 
  
   1.30  | 
 
| 
   10.0  | 
  
   1.23  | 
            
        WA20738.087, June 2002