• Application Note

Gradient Separation of Nutrients on XBridge HILIC

Gradient Separation of Nutrients on XBridge HILIC
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application note demonstrates the gradient separation of nutrients on XBridge HILIC.

Introduction

The compounds used in this study are:

  1. Nicotinamide
  2. Nicotinic acid
  3. Thiamine
Compounds

Experimental

Method Conditions

Column:

XBridge HILIC, 2.1 x 50 mm, 3.5 μm

Part Number:

186004432

Mobile Phase A:

10 mM NH4COOH in H2O, 0.125% HCOOH in 50:50 ACN:H2O

Mobile Phase B:

10 mM NH4COOH in H2O, 0.125% HCOOH in 90:10 ACN:H2O

Flow Rate:

0.6 mL/min

Injection Volume:

5 μL

Sample Concentration:

25 μg/mL each

Sample Diluent:

75:25 ACN:MeOH with 0.2% HCOOH

Column Temperature:

30 °C

Detection:

UV @ 268 nm

Sampling Rate:

40 points/sec

Filter Time Constant:

0.1

Instrument:

Waters ACQUITY UPLC with ACQUITY PDA

Gradient

Gradient

Results and Discussion

Results

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WA64075, August 2009

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