Aflatoxins in Peanuts

Abstract

This application brief describes the analysis of alfatoxins in peanuts.

This application brief describes the analysis of alfatoxins in peanuts.

1. Add 5 g of sodium hydroxide to 20 g of homogenized sample, followed by 30 mL of n-hexane.

2. Add 100 mL 60% aqueous methanol and homogenize.

3. Ultrasonicate for 30 minutes.

4. Filter sample through 15 cm filter paper.

5. Take 1 mL aliquot from 60% methanol layer for SPE cleanup.

Instrument:	Alliance HPLC 2695 System
Column:	Symmetry Shield RP18, 4.6 x 150 mm, 5 μm
Flow rate of iodine:	0.2 mL
Flow rate:	1 mL/min
Mobile phase:	A. methanol B. water
Isocratic gradient:	35% A: 65% B, for 20 minutes
Column temperature:	30 °C
Derivatization temp.:	80 °C
Excitation wavelength:	365 nm
Emission wavelength:	455 nm
Post-column derivatizaton reagent:	Dissolve 200 mg iodine in 10 mL methanol, top up 1000 mL with water
Detector:	2475 Multi Wavelength Fluorescence

Matrix interference is greatly reduced when sample is cleaned up by using Oasis HLB SPE cartridge.

Results of B1, B2, G1, G2 in peanuts (n=5)