• Application Note

Hydrophobic Bases: Group of Antihistamines

Hydrophobic Bases: Group of Antihistamines
  • Waters Corporation
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Hexagon molecule structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of hydrophobic bases.

Introduction

Compounds used in this application brief are shown below.

Experimental

Conditions

Column:

SunFire C18 4.6 x 150 mm, 5.0 μm (p/n: 186002559)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

1% HCOOH in water

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water;

Diphenhydramine: 50 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Conditions

Column:

SunFire C8 4.6 x 150 mm, 5.0 μm (p/n: 186002737)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

1% HCOOH in water

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water;

Diphenhydramine: 50 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Compounds

USP Tailing

Chlorpheniramine

1.01

Triprolidine

1.1

Diphenhydramine

2.17

Conditions

Column:

SunFire C8 4.6 x 100 mm, 5.0 μm (p/n: 186002731)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

1% HCOOH in water

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water;

Diphenhydramine: 50 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Results and Discussion

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WA41892, May 2005

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