• Application Note

Tetracyclines in Milk (Antibacterials)

Tetracyclines in Milk (Antibacterials)
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of tetracyclines in milk.

Introduction

The compound analyzed in this study is tetracyclines in milk.

Experimental

The milk sample (15 mL) is diluted with 25 mL of McIlvaine (mixed citrate/phosphate, pH 4.1) buffer with added EDTA. The diluted sample is centrifuged at 8000 x g for 10 minutes at 5° C. Any floating lipid layer is removed and the remaining supernatant is processed using Oasis HLB solid-phase extraction cartridges.

For details of the buffer preparation procedure see J.AOAC Int., 79 (2), p 405 (1996).

HPLC Method

Column:

Nova-Pak C8, 3.9 x 150 mm, 4 μm

Part number:

WAT086344

Mobile phase:

ACN, Methanol, 50 mm oxalic acid in water; 13:13:74

Flow rate:

0.8 mL/min

Injection volume:

60 μL

Detection:

UV @ 365 nm

Results and Discussion

Featured Products

WA31764.150 , June 2003

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