• Application Note

Sulfamethoxazole and Trimethoprim

Sulfamethoxazole and Trimethoprim
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of Sulfamethoxazole and Trimethoprim using Symmetry Columns.

Introduction

Compounds used in this study are:

1. Trimethoprim

2. Sulfamethoxazole

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 20 mm, 5 μm IS (p/n: WAT046980)

Mobile phase:

Water/methanol/glacial acetic acid 79:20:1

Flow rate:

1.0 mL/min

Injection volume:

10 μL of 100 μg/mL sulfamethoxazole and 30 μg/mL trimethroprim

Detection:

UV @ 254 nm

USP Tailing Factors

1. 1.5

2. 1.1

Results and Discussion

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WA31763.158, June 2003

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