• Application Note

Steroids - 4.6 x 20 mm Intelligent Speed Separation

Steroids - 4.6 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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Prednisone pills and bottle pour out on table

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of steroids using 4.6 x 20 mm Intelligent Speed Separation Column.

Introduction

The compounds used in this study are - 

  1. Prednisone
  2. Cortisone
  3. Corticosterone
  4. Estradiol
  5. Estrone
  6. Progesterone

Experimental

HPLC Method

Column:

Symmetry C18, 4.6 x 20 mm, 3.5 μm IS

Symmetry Shield RP18, 4.6 x 20 mm, 3.5 μm IS

Part numbers:

Symmetry - 186002090

Symmetry Shield - 186002092

Mobile phase A:

Water

Mobile phase B:

ACN

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Temperature:

30 °C

Detection:

UV @ 220 nm

Gradient

Gradient.

Results and Discussion

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WA31763.152, June 2003

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