• Application Note

Chlordiazepoxide and Metabolites

Chlordiazepoxide and Metabolites
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of chlordiazepoxide and metabolites.

Introduction

The compounds used in this study are -

Compounds

USP Tailing Factors

Norchlordiazepoxide

1.1

Oxazepam

1.2

Nordiazepam

1.1

Chlordiazepoxide

1.2

Demoxepam

1.1

Experimental

HPLC Method

Column:

Symmetry C18, 3.0 x 150 mm, 5 μm

Part number:

WAT054200

Mobile phase:

20 mM potassium phosphate pH 7.0/methanol/acetonitrile 49:28:23

Flow rate:

0.59 mL/min

Injection volume:

5 μL of 40 74 μg/mL

Detection:

UV @ 240 nm

Results and Discussion

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WA31763.53, June 2003

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