This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of acetaminophen and hydrocodone bitartrate using Symmetry C18 Columns.
Coumpounds used in this study are:
1. Acetaminophen
2. Hydrocodone Bitartrate
Column: |
Symmetry C18, 3.9 x 150 mm, 5 μm (p/n: WAT046980) |
Mobile phase: |
50 mM Ammonium phosphate, pH 5.8/acetonitrile 85:15 |
Flow rate: |
1.0 mL/min |
Injection volume: |
20 μL of 775 μg/mL acetaminophen and 8 μg hydrocodone bitartrate sample |
Detection: |
UV @ 214 nm |
WA31763.12, June 2003