• Application Note

Sulfonamides

Sulfonamides
  • Waters Corporation
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Capsule pills in hand with water in glass

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of Sulfonamides using SunFire C18 and C8 Columns. Sulfonamides are class of antibiotics used to treat a wide range of bacterial infections.

Introduction

The compounds analysed in this study using SunFire C18 Column are -

The compounds analysed in this study using SunFire C8 150 mm Column are -

The compounds analysed in this study using SunFire C8 100 mm Column are -

Experimental

Conditions

Column:

SunFire C18, 4.6 x 150 mm, 5 μm

Part number:

186002559

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

100 mM CH3COO-NH4+, pH 5.0

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Gradient.

Conditions

Column:

SunFire C8, 4.6 x 150 mm, 5 μm

Part number:

186002737

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

100 mM CH3COO-NH4+, pH 5.0

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Note: Method is same as for the C18.

Gradient

Gradient.

Conditions

Column:

SunFire C8, 4.6 x 100 mm, 3.5 μm

Part number:

186002731

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

100 mM CH3COO-NH4+, pH 5.0

Flow rate:

1 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Gradient.

Results and Discussion

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WA41902, May 2005

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