• Application Note

Steroids - Symmetry C8 Column

Steroids - Symmetry C8 Column
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of steroids using Symmetry C8 Column.

Introduction

The compounds used in this study are – 

Compounds

USP Tailing Factors

Prednisolone

1.08

Betamethasone

1.18

Prednisolone Acetate

1.1

Betamethasone Valerate

1.31

Experimental

HPLC Method

Column:

Symmetry C8 ,3.9 x 150 mm, 5 μm

Guard column:

Sentry Guard Column 3.9 x 20 mm, 5μm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

Acetonitrile/water 50:50

Flow rate:

1.0 mL/min

Injection volume:

5μL of 10 μg/mL sample

Detection:

UV @ 254 nm

Results and Discussion

Featured Products

WA31763.149, June 2003

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