• Application Note

Tricyclic Antidepressants - Isocratic, pH 7.0, XTerra RP18 

Tricyclic Antidepressants - Isocratic, pH 7.0, XTerra RP18 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of tricyclic antidepressants using XTerra RP18 Columns. 

Introduction

The compounds analyzed in this study are: 

  1. Nordoxepin 
  2. Desipramine 
  3. Nortriptyline 
  4. Doxepin 
  5. Imipramine 
  6. Amitriptyline 
  7. Trimipramine
Compounds

Experimental

Conditions

Column:

XTerra RP18 4.6 x 100 mm, 3.5 µm

Part number:

186000438

Mobile phase A:

10 mM NH4COOH, pH 3.8

Mobile phase B:

ACN

Flow rate:

1.5 mL/min

Isocratic mobile phase composition:

57% A; 43% B

Injection volume:

10 µL

Temperature:

50 °C

Detection:

UV @ 250 nm

Instrument:

Alliance 2695, 2996 PDA
USP Tailing

Results and Discussion

Results

Featured Products

WA20738.108, June 2002

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