UPLC Column 2.1 x 150 mm

SKU: 186008756
ACQUITY UPLC Peptide HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

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Product Description

ACQUITY UPLC Peptide HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1K - 15K Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods.

Waters is phasing out Certificate of Analysis (COAs) for eCords. All column documents are now available at www.waters.com/coa

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    T3

  • Silanol Activity

    Medium

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    15000

  • Particle Shape

    Spherical

  • Particle Size

    1.8 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    100 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    230

  • System

    UPLC, UHPLC

  • Particle Technology

    HSS

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    150 mm

  • Carbon Load

    11 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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What Is Sensitivity Within Chromatography?

Sensitivity refers to the signal output per unit concentration or unit mass of a substance in the mobile phase entering the detector, as an example, the slope of a linear calibration curve. Sensitivity also refers to the ratio of peak height to the analyte concentration in the peak for concentration-sensitive detectors. It is the ratio of peak height to unit mass in mass-flow-sensitive detectors.

What Are Some Of The System Limits Using Acquity UPLC Peptide HSS T3 Column?

The Acquity UPLC Peptide HSS T3 Column can be operated between a pH range of 2 - 8, with a temperature ceiling of 45 C, and up to a maximum pressure of 15,000 psi (or 1034 Bar).