• Application Note

Tricyclic Antidepressants in Plasma

Tricyclic Antidepressants in Plasma
  • Waters Corporation
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Plasma preparation for centrifuge

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of tricyclic antidepressants in plasma.

Introduction

The compounds used in this study are – 

Spiked at 500 ng/mL versus Blank:

  1. Nordoxepin (I.S.)
  2. Nortriptyline
  3. Doxepin
  4. Imipramine
  5. Amitriptyline
  6. Trimipramine

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 4.6 x 75 mm, 3.5 μm

Part numbers:

WAT094263

Mobile phase:

50 mM phosphate, pH 7/methanol 26:74

Flow rate:

1.4 mL/min

Injection volume:

1 mL spiked plasma plus 20 μL concentrated phosphoric acid, 100 μL injection

Temperature:

29 °C

Detection:

UV @ 254 nm

Results and Discussion

Chromatogram of Plasma Extracts: A) Blank B) Spiked Sample

Chromatogram of Plasma Extracts: A) Blank B) Spiked Sample

Featured Products

WA31763.175, June 2003

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