• Application Note

Drugs of Abuse, pH 10.0 – 2.1 x 20 mm Intelligent Speed Separation

Drugs of Abuse, pH 10.0 – 2.1 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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Loaded syringe and pills

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of drugs of abuse.

Introduction

The compounds used in this study are –

Experimental

Conditions

Column:

Xterra RP18, 2.1 x 20 mm IS, 3.5 μm, (p/n: 186001925)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

100 mM NH4HCO3, pH 10

Flow rate:

0.8 mL/min

Injection volume:

5 μL

Sample concentration:

MDA and MDMA 40 μg/mL, all others 20 μg/mL

Temperature:

50 °C

Detection:

UV @ 235 nm

Instrument:

Alliance 2795 with 996 PDA

Gradient

Gradient.

Results and Discussion

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WA31787.13, June 2003

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