• Application Note

Clemastine in Rat Plasma - Oasis On-Line (2 Column Approach (Using XTerra Oasis HLB Extraction Column 2.1 × 20 mM, 25 mM, XTerra Analytical Column 2.1 × 30, 3.5 mM) 

Clemastine in Rat Plasma - Oasis On-Line (2 Column Approach (Using XTerra Oasis HLB Extraction Column 2.1 × 20 mM, 25 mM, XTerra Analytical Column 2.1 × 30, 3.5 mM) 

  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of clemastine in rat plasma using Oasis HLB extraction method.

Introduction

Clemastine has been studied in this application brief.

Experimental

Conditions

LC1:

Alliance 2790 - 0.4 mL/min

LC2:

Waters 515–4.0 mL/min

Loading mobile phase:

100% water

Eluting mobile phase:

1 minute gradient 5% ACN to 95% ACN

Eluting mobile phase additive:

0.5% Formic acid

Extraction column temp.:

40 °C

Switching valve:

Rheodyne LabPro 10 ports, 2 position

MS:

Waters Micromass Quattro Ultima Triple Quadrupole

Ion source:

ESI+

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar

Desolvation gas:

600 L/hr

Cone voltage:

20 V

Collision energy:

20

Gradient

A - Acetonitrile + 0.5% Formic Acid

B - Water + 0.5% Formic Acid

Results and Discussion

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WA31764.56, June 2003

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