• Application Note

Desalting of Peptide Mixture Prior to ESI-MS Analysis

Desalting of Peptide Mixture Prior to ESI-MS Analysis

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of peptide mixture.

Introduction

Experimental

EST-MS Conditions

Waters Micromass LCT System

Capillary voltage:

2.8 kV

Sample cone voltage:

35 V

Extraction cone voltage:

5 V

Gas flow:

520 and 310 L/h

Peptide mixture prepared by digestion of Cytochrome c by trypsin in 20 mM trisglycine buffer.

References

  1. M. Gilar, A. Belenky, B. H. Wang, J. Chromatog. A, 921 (2001) 3–13.

Featured Products

WA31764.66, June 2003

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