This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates benefits of using ACQUITY UPLC combined with an ACQUITY QDa Detector for rapid raw material testing of cetrimonium bromide, instead of a multistep titrimetric assay.
The UPLC method coupled with an ACQUITY QDa Detector eliminates titrimetric analysis with a complex sample preparation, resulting in time savings and increased productivity.
Pharmaceutical raw materials are substrates or elements used for manufacturing of different drug products. Raw materials include active pharmaceutical ingredients (API), excipients, and other inactive ingredients. While they generally have no pharmacological effect, excipients are essential components that function as fillers, binders, disintegrants, lubricants, coloring agents, and preservatives.1
Cetrimonium bromide (CTAB) is an antiseptic agent used for prevention of bacteria and fungi growth. It can also be used as a cosmetic biocide, surfactant, and preservative.2 The method listed in the United States Pharmacopeia (USP) Monograph for Cetrimonium Bromide3 is based on a titrimetric analysis of a sample solution prepared using a multistep liquid-liquid extraction with chloroform. This is a complex and time-consuming procedure, not ideal for routine assay testing within a QC laboratory.
This method was developed for use by a large biopharmaceutical company to develop a robust and quick UPLC method for a cetrimonium bromide assay to replace a multistep titrimetric assay method. This application brief illustrates the use of UPLC coupled with an ACQUITY QDa – a small and robust mass detector for rapid raw material testing.
Sample used in this study was prepared by dissolving cetrimonium bromide in water to make a stock concentration of 0.4 mg/mL. Stock solution was then diluted with water to the working concentration of 2.5 μg/mL.
The chromatographic separation was performed on an ACQUITY UPLC H-Class System using method conditions described in Figure 2.
Since cetrimonium bromide lacks chromophores required for UV detection, it cannot be directly detected by UV. However, it is readily ionizable, producing a robust MS signal on the ACQUITY QDa Detector. Figure 3 shows several traces for cetrimonium bromide. As expected, no peaks were detected on the UV trace at 215 nm (Figure 3a). The ACQUITY QDa data collected across the entire mass range (150 – 350 Da, Figure 3b) is referred as the total ion chromatogram (TIC). The TIC can be extracted at mass 284.3 Da to give an extracted ion chromatogram (XIC) specific to the target of interest as illustrated in Figure 3c. For targeted analyses and a targeted assay desired, data can be collected simply using the single ion recording (SIR) scanning mode, which only scans for the peak of interest and can greatly simplify analysis as well as increase S/N and sensitivity further (Figure 3d.). The ACQUITY QDa Detector enabled direct detection of nonchromophoric cetrimonium bromide without the need for a complex sample preparation procedure.
The performance of the developed UPLC method was verified by evaluating repeatability of six replicate injections of the 2.5 μg/mL standard according to the specifications defined in the USP General Chapter, <621>, Chromatography.4 The UPLC System suitability results were processed using SIR mass data at 284.3 Da (Figure 4). The retention times and area repeatability were well within the USP specifications of less than 2% RSD.
Linearity of the method for cetrimonium bromide with SIR mass detection at 284.3 Da was evaluated over 7 concentrations ranging from 0.1 μg/mL to 3 μg/mL. The method showed a good linear behavior between the peak areas and concentrations of centrimonium bromide with correlation coefficient (R2) ≥0.999 (Figure 5)
The UPLC-MS method developed with ACQUITY UPLC and ACQUITY QDa eliminates a complex sample preparation procedure and enables a quick and easy assay of the raw material with confirmation and quantitation. This resulted in time savings and increased productivity. In addition, system suitability and linearity of the method calculated using mass data were excellent.
Overall, the ACQUITY QDa Detector is a robust and simple-to-use mass detector that can be easily added as an orthogonal detection technique. It provides accurate and reliable results, making this technology ideal for routine testing in the QC laboratory.
720005265, January 2015