Analysis of Food Sugars/Saccharides in Honey Using ACQUITY UPLC BEH Amide Columns

Column:

ACQUITY UPLC BEH Amide 2.1 x 50 mm, 1.7 μm

Part Number:

186004800

Mobile Phase A:

80/20 MeCN/H₂O with 0.2% triethylamine [TEA]

Mobile Phase B:

30/70 MeCN/H₂O with 0.2% triethylamine [TEA]

Flow Rate:

0.15 mL/min

Injection Volume:

0.7 μL (PLNO)

Sample Concentration:

Honey and corn syrup at 5-10 mg/mL each

Sample Diluent:

50/50 MeCN/H₂O

Column Temperature:

45 °C

Strong Needle Wash:

20/80 MeCN/H₂O (800 μL)

Weak Needle Wash:

75/25 MeCN/H₂O (500 μL)

Seal Wash:

50/50 MeCN/H₂O

Instrument:

Waters ACQUITY UPLC with ELSD

Gradient:

5 minute gradient, 80%-50% MeCN (w/0.2% TEA) with 10 minute re-equilibration

Gain:

200

Pressure:

40 psi

Drift Tube Temperature:

40 °C

Nebulizer:

Cooling

Data Rate:

10 pps

Filter Time Constant:

Normal