This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of steroids in formic acid.
The compounds used in this study are -
            
        | 
                     Column:  | 
                     SunFire C18 4.6 x 150 mm, 5 μm  | 
            |
| 
                     Part number:  | 
                     186002559  | 
            |
| 
                     Mobile phase A:  | 
                     0.1% HCOOH in Water  | 
|
| 
                     Mobile phase B:  | 
                     0.1% HCOOH in ACN  | 
|
| 
                     Flow rate:  | 
                     1.4 mL/min  | 
            |
| 
                     Injection volume:  | 
                     10 μL  | 
            |
| 
                     Sample concentration:  | 
                     50 mg/mL each, in DMSO  | 
            |
| 
                     Temperature:  | 
                     Ambient  | 
            |
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            |
| 
                     Instrument:  | 
                     AutoPurification System  | 
            
            
        
            
        WA41899, March 2005