• Application Note

Anilines, pH 10.0 – 3.0 x 20 mm Intelligent Speed Separation

Anilines, pH 10.0 – 3.0 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of anilines using XTerra Columns. 

Introduction

The compounds analyzed in this study are: 

  1. Caffeine 
  2. Aniline 
  3. N-Methylaniline 
  4. 2-Ethylaniline 
  5. 4-Nitroanisole 
  6. N,N-Dimethylaniline  

Experimental

Conditions

Column:

XTerra MS C18, 3.0 x 20 mm IS, 3.5 μm, (P/N: 186001974)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

100 mM NH4HCO3, pH 10

Flow rate:

2.0 mL/min

Injection volume:

5.0 μL

Sample concentration:

20 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Gradient

Results and Discussion

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WA31787.11, June 2003

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