The XBridge Premier Protein BEH C4 300 Å 2.5 µm, 4.6 x 100 mm columns are QC tested with the MassPREP Protein Mix standard (p/n: 186004900) and are optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.
Note: The same column is also available with the MassPREP Protein Mix Standard at no additional cost (p/n: 176005114)
Suggested use: Low-level intact protein and subunit analysis, phosphorylated proteins. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software.
Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
10 pH |
Maximum Pressure |
10000 psi (690 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Protein |
Format |
Premier Column |
Surface Area |
90 |
System |
UHPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L26 |
Inner Diameter |
4.6 mm |
Length |
100 mm |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
eConnect |
Yes |