ACQUITY UPLC I-Class/Xevo TQD IVD System: Analytical Performance for a Thymidylate Synthase Inhibitor
For in vitro diagnostic use. Not available in all countries.
Introduction
The Waters ACQUITY UPLC I-Class/Xevo TQD IVD System enables the quantification of organic compounds in human biological liquid matrices.
This document describes a test of the analytical performance of the ACQUITY UPLC I-Class/Xevo TQD IVD System for the analysis of 5-fluorouracil in plasma.
Experimental
The ACQUITY UPLC I-Class/Xevo TQD IVD System was controlled by MassLynx IVD (v4.1) and the data processed using the TargetLynx Application Manager. Calibrators and Quality Controls were prepared by spiking commercially available reference material in plasma and the samples were processed using the following conditions:
Sample Preparation Conditions
50 μL sample was processed in a liquid-liquid extraction with acidified ethyl acetate followed by a solvent exchange to 0.1% formic acid in water prior to analysis.
LC Conditions
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Column: |
ACQUITY UPLC HSS PFP 1.8 μm, 2.1 mm × 100 mm |
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Mobile phase A: |
Water |
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Mobile phase B: |
Acetonitrile |
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Flow rate: |
0.4 mL/min |
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Gradient: |
2% B for 0.5 minutes, 2–60% B over 1.5 minutes, 98% B for 0.5 minutes, 98% A for 0.5 minutes |
MS Conditions
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Resolution: |
MS1 (0.75 FWHM), MS2 (0.75 FWHM) |
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Acquisition mode: |
MRM |
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Polarity: |
ESI (-) |
Results and Discussion
Performance characteristics of 5-fluorouracil on the ACQUITY UPLC I-Class/Xevo TQD IVD System are shown in Table 1. A chromatogram illustrating the analytical sensitivity of the 5-fluorouracil analysis is shown in Figure 1.
Conclusion
The Waters ACQUITY UPLC I-Class/Xevo TQD IVD System has demonstrated the capability to deliver analytical sensitivity and precision for the analysis of 5-fluouroracil in plasma.
Disclaimer
The analytical performance data presented here is for illustrative purposes only. Waters does not recommend or suggest analysis of the analytes described herein. These data are intended solely to demonstrate the performance capabilities of the system for analytes representative of those commonly analyzed using liquid chromatography and tandem mass spectrometry. Performance in an individual laboratory may differ due to a number of factors, including laboratory methods, materials used, intra-operator technique, and system conditions. This document does not constitute a warranty of merchantability or fitness for any particular purpose, express or implied, including for the testing of the analytes in this analysis.
720006305, Revised June 2023
