• Application Note

Terfenadine and Metabolites by Reverse-Phase SPE and LC-MS/MS

Terfenadine and Metabolites by Reverse-Phase SPE and LC-MS/MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of terfenadine and metabolites by reverse-phase SPE and LC-MS/MS.

Introduction

The compounds analyzed in this study are terfenadine and metabolites.

Experimental

HPLC Method

Column:

Xterra MS C18, 2.1 x 30 mm, 3.5 μm

Part number:

186000398

Mobile phase A:

Water + 0.1M NH4 formate pH 9.5

Mobile phase B:

MeOH + 0.1M NH4 formate pH 9.5

Flow rate:

0.4 mL/min

Temperature:

Ambient

LC instrument:

Alliance 2795

Gradient

Gradient.

MS Conditions

MS instrument:

Waters Micromass Quattro

Ion source:

ESI+

Source temperature:

150 °C

Gas cell:

2.0e-3 bar Argon

Cone voltage:

35 volts

Capillary voltage:

3.5 kV

Drying gas flow:

500 L/hr

Cone gas flow:

50 L/hr

Desolvation temperature:

350 °C

MRM transition:

Protriptyline (IS) m/z 263.9 → 190.8

Terfenadine m/z 472.2 → 436.2

Terfenadine-alcohol m/z 488.2 → 452.2

Terfenadine-carboxylate m/z 502.2 → 466.2

Results and Discussion

Method Validation of Terfenadine and Metobolites on Oasis HLB μElution Plate

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WA31764.144 , June 2003

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