• Application Note

Methoxyverapamil- pH 2.5, LC-MS

Methoxyverapamil- pH 2.5, LC-MS
  • Waters Corporation
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Verapamil calcium channel blocker drug molecule structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application note details on the analysis of methoxyverapamil using XTerra MS C18 Columns. 

Introduction

The compound analyzed in this study is methoxyverapamil.  

Methoxyverapamil

Experimental

LC Conditions

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 µm

Part number:

186000398

Mobile phase A:

0.1% HCOOH in H2O, pH 2.5

Mobile phase B:

0.1% HCOOH in ACN, pH 2.5

Flow rate:

0.2 mL/min to MS

Isocratic mobile phase composition:

75%A; 25%B

Injection volume:

20 µL of 100 pg/µL

Temperature:

Ambient

Detection:

MS ESI+, SIR 485.4

Instrument:

Alliance 2795 HT, Micromass ZQ

MS Conditions

Micromass ZQ

ESI+

Capillary (KV):

3.0

Cone (V):

45

Extractor:

3.0

RF lens:

0.5

Source temp.:

150

Desolvation temp.:

350

Cone gas flow (L/Hr):

60

Desolvation gas flow (L/Hr):

500

LM resolution:

15

HM resolution:

15

Ion energy:

1.0

Multiplier (V):

650

Results and Discussion

Results

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