• Application Note

Diuretics - Gradient, pH 3.6

Diuretics - Gradient, pH 3.6
  • Waters Corporation
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Furosemide prescription bottle with stethoscope

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of diuretics.

Introduction

The compounds used in this study are –

  1. Triamterene
  2. Chlorthalidone
  3. Althiazide
  4. Furosemide
  5. Benzthiazide
  6. Probenecid
  7. Ethacrynic acid
  8. Bumetanide
  9. Canrenoic acid

Experimental

Conditions

Column:

Xterra MS C18 4.6 x 50 mm, 3.5 μm

Part number:

186000432

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

100 mM NH4COOH, pH 3.6

Flow rate:

2.0 mL/min

Injection volume:

20 μL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient

Gradient.

Results and Discussion

Featured Products

WA20738.041, June 2002

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