• Application Note

Alprenolol - Isocratic, LC-UV

Alprenolol - Isocratic, LC-UV
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of alprenolol.

Introduction

The compound analyzed in this study is alprenolol.

Mobile Phase pH

USP Tailing

3.0

1.19

7.0

1.64

10.0

1.08

Experimental

Conditions

Column:

Xterra RP18 4.6 x 50 mm, 5 μm

Part number:

186000492

Mobile phase:

pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 40:50:10

pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 55:35:10

pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 55:35:10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30 °C

Detection:

UV @ 270 nm

Instrument:

Alliance 2695, 2996 PDA

Results and Discussion

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WA20738.007, June 2002

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