• Nota de aplicación

Prednisolone

Prednisolone
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of prednisolone using XTerra RP18 columns.

Introduction

Prednisolone is studied in this application brief.

Experimental

Conditions

Column:

XTerra RP18 4.6 x 150 mm, 5 μm (p/n: 186000492)

Mobile phase:

H2O/ACN/100 mM NH4COOH, pH 3.0, 35: 55: 10

H2O/ACN/100 mM NH4HCO3, pH 7.0, 35: 55: 10

H2O/ACN/100 mM NH4HCO3, pH 10.0, 58: 32: 10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30°C

Detection:

UV @ 270 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile phase pH

USP Tailing

3.0

1.19

7.0

1.21

10.0

1.12

 

Results and Discussion

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WA20738.090, June 2002

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