ACQUITY UPLC I-Class System: Repeatable Chromatographic Performance with 1.0 mm Diameter Columns
This is an Application Brief and does not contain a detailed Experimental section.
Abstract
The ACQUITY UPLC I-Class System produces high-quality UPLC Chromatography with 1.0 mm Diameter Columns.
Benefits
Introduction
Reducing the amount of sample required for an analysis, gaining a boost in sensitivity, or decreasing solvent usage and waste generation are some of the reasons laboratories may want to migrate their analyses to 1.0 mm diameter columns. To achieve these benefits, however, highly repeatable chromatography must be demonstrated. Replicate injections of a small molecule standard were analyzed on an ACQUITY UPLC I-Class System with UV detection using a 1.0 mm Column to evaluate system performance.
Experimental
LC Conditions
|
Sample: |
6-component small molecule standard in 30% acetonitrile/water |
|
Mobile phase: |
A – 0.1% formic acid in water, B – 0.1% Formic acid in Acetonitrile |
|
Flow rate: |
90 µL/min |
|
Gradient: |
5% to 95% B, 25 min |
|
Column: |
HSS T3 1.0 x 150 mm, 1.8 µm |
|
Wavelength: |
270 nm, 10 Hz, normal filter Column |
|
Temperature: |
40 °C |
|
Injection vol: |
1 µL |
Results and Discussion
The results for five replicate injections are shown in the chromatogram and table below. The overlay plot was offset to more clearly show the consistent chromatography. UPLC peak widths were evident, ranging from 5.2 to 9.0 seconds at 10% peak height. Retention time %RSD values were all 0.11% or less. And peak area repeatability for all peaks was ≤0.20% RSD.
Conclusion
The ACQUITY UPLC I-Class System produces high-quality UPLC Chromatography with 1.0 mm Diameter Columns.
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720005305, January 2024
