• Application Note

Isocratic Analysis of Amoxicillin Using Atlantis T3 Columns

Isocratic Analysis of Amoxicillin Using Atlantis T3 Columns
  • Waters Corporation
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Pharmacist holding antibiotic capsule pack in pharmacy

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates isocratic analysis of amoxicillin using Atlantis T3 Columns.

Introduction

The compound used in this study is amoxicillin.

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 50 mm, 3 μm

Part number:

186003726

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM HCOONH4, pH 3.0

Flow rate:

1.0 mL/min

Isocratic mobile phase composition:

84% A; 6% B; 10% C

Injection volume:

20 μL

Sample:

Amoxicillin (40 μg/mL) in H2O

Column temperature:

30 °C

Sample temperature:

20 °C

Detection:

UV @ 254 nm

Sampling rate:

5 point/sec

Filter response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Results and Discussion

Featured Products

WA60215, January 2009

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