• Application Note

Gradient Separation of Bamethan and Albuterol on ACQUITY UPLC BEH HILIC

Gradient Separation of Bamethan and Albuterol on ACQUITY UPLC BEH HILIC
  • Waters Corporation
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Asthma medication albuterol inhaler

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the gradient separation of bamethan and albuterol on ACQUITY UPLC BEH HILIC Columns.

Introduction

The compounds used in this study are:

  1. Bamethan
  2. Albuterol

Experimental

Test Conditions

Column:

ACQUITY UPLC BEH HILIC, 2.1 x 100 mm, 1.7 µm

Part Number:

186003461

Mobile Phase A:

10 mM NH4COOH, 0.2% HCOOH in 90:10 ACN:H2O

Flow Rate:

0.708 mL/min

Isocratic Mobile Phase Composition:

100% A

Injection Volume:

0.8 µL

Sample Concentration:

125 µg/mL

Sample Diluent:

75:25 ACN:MeOH with 0.2% HCOOH

Temperature:

30 °C

Detection:

UV @ 280 nm

Sampling Rate:

20 pts/sec

Time Constant:

0.1

Instrument:

Waters ACQUITY UPLC with ACQUITY TUV

Results and Discussion

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WA60135, August 2009

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