• Application Note

Sulfonamide Antibacterials in Milk

Sulfonamide Antibacterials in Milk
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief describes the process of monitoring the presence of sulfonamides in milk.

Introduction

Sulfonamides are widely used for therapeutic and prophylactic purposes in animals. When sulfonamides are retained in food stuff, this may result in allergic or toxic reactions in sensitive consumers. This method can be used to monitor the presence of sulfonamides in milk.

Experimental

SPE Procedure

LC Conditions

System:

ACQUITY UPLC System

Column:

ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 100 mm

Flow rate:

0.2 mL/min

Mobile phase A:

0.05% formic acid/water

Mobile phase B:

0.05% formic acid/methanol

Detector:

ACQUITY UPLC PDA Detector

Gradient

MS Conditions

MS System:

Waters Quattro Premier XE

Ionization mode:

Positive electrospray (ESI+)

Multiple reaction monitoring
MRM method parameters. 

Results and Discussion

Whole milk (5 ng/mL) typical LC-MS/MS analysis (MRM). 

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720002588, April 2008

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