This application brief highlights the determination of formaldehyde in ambient air using adsorbant cartridge followed by
High Performance Liquid Chromatography
Formaldehyde is an important industrial chemical used in the manu-facturing of other chemicals, building materials, and household products. It is one of the large family of chemical compounds called volatile organic compounds or “VOCs”. At normal room temperatures these compounds vaporize. When present in air at levels above 0.1 ppm it can cause watery eyes, burning sensations in the eyes and nasal passages, as well as coughing, wheezing, and allergic reactions. Formaldehyde has been classified as a potential carcinogen and, as such, is regulated in many countries: Japan, 0.08 ppm; World Health Organization Europe, 0.08 ppm; Sweden, 0.1 ppm; US Department of Housing and Urban Development, 0.4 ppm.
Instrument: |
Waters Alliance HPLC system with UV detection |
Eluent: |
Water/tetrahydrofuran/acetonitrile |
Column: |
Waters XBridge Phenyl, 3.5 μm, 4.6 x 150 mm @ 35 °C |
Injection: |
20 μL each of AccuStandard mix (M- 8315-R1- DNPH and M- 8315-R2- DNPH) diluted 1:5 in 40:60 water/acetonitrile |
Flow Rate: |
1.5 mL/min |
Detection: |
UV @ 360 nm |
Data: |
Waters Empower software |
Use Sep-Pak DNPH Silica cartridge, backflush cartridge with acetonitrile.
Filter and degas through a 0.45 μm filter.
A: 90% water, 10% tetrahydrofuran (THF). Mix 900 mL water and 100 mL stabilized THF.
B: Acetonitrile
Time |
Flow |
%A |
%B |
Curve |
---|---|---|---|---|
Initial |
1.5 |
70 |
30 |
- |
20 |
1.5 |
36 |
64 |
6 |
22 |
1.5 |
36 |
64 |
6 |
22.1 |
1.5 |
70 |
30 |
6 |
Time |
Flow |
%A |
%B |
Curve |
---|---|---|---|---|
Initial |
1.5 |
70 |
30 |
- |
16 |
1.5 |
53 |
47 |
6 |
21 |
1.5 |
53 |
47 |
6 |
21.1 |
1.5 |
70 |
30 |
6 |
720002738, 2008