• Application Note

Steroids in Formic Acid

Steroids in Formic Acid

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of steroids in formic acid.

Introduction

The compounds used in this study are - 

  1. Prednisolone
  2. Dexamethasome
  3. Betamethasone 17-valerate
  4. Progesterone

Experimental

Column:

SunFire C18 4.6 x 150 mm, 5 μm

Part number:

186002559

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in ACN

Flow rate:

1.4 mL/min

Injection volume:

10 μL

Sample concentration:

50 mg/mL each, in DMSO

Temperature:

Ambient

Detection:

UV @ 254 nm

Instrument:

AutoPurification System

Gradient

Results and Discussion

Featured Products

WA41899, March 2005

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