This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the analysis of diazepam by LC-MS.
The compound analyzed in this study is diazepam.
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Column: |
Symmetry C18, 2.1 x 100 mm, 3.5 μm |
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Part number: |
WAT058965 |
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Mobile phase: |
2 mM Ammonium Acetate/Acetonitrile/Formic Acid 65:35:0.1 |
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Flow rate: |
200 μL/min |
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Injection volume: |
10 μL |
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MS: |
Micromass Quattro LC |
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Ion Mode: |
ES+ |
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Cone Voltage: |
45 V |
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Collision Energy: |
25 eV |
Figure 1. Background-subtracted electrospray mass spectrum of pure diazepam standard (5μg), under optimum conditions. Chromatographed as described above to remove contaminants.
Figure 2. Background-subtracted electrospray product tion spectrum of pure diazepam standard (5μg), under optimum conditions. Chromatographed as described above to remove contaminants.
Figure 3. Calibration curve with triplicate injections for each point and demonstrating LOQ at 0.2ng/mL.
Figure 4. MRM Chromatograms under optimum conditions of pure diazepam standard at (A) 0.2ng/mL (LOD) and (B) 5.0ng/mL and (C) a processed human plasma sample with a low concentration of diazepam (calculated as 0.75ng/mL).
WA31763.72, June 2003
