• Application Note

Gradient Separation of Guanine and Acyclovir on ACQUITY UPLC BEH HILIC

Gradient Separation of Guanine and Acyclovir on ACQUITY UPLC BEH HILIC
  • Waters Corporation
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Acyclovir is a guanosine analogue antiviral medication

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

Thia application note demonstrates the gradient separation of guanine and acyclovir on ACQUITY UPLC BEH HILIC Columns.

Introduction

The compounds used in this study are:

  1. Acyclovir
  2. Guanine

Experimental

Test Conditions

Column:

ACQUITY UPLC BEH HILIC, 2.1 x 100 mm, 1.7 μm

Part Number:

186003461

Mobile Phase A:

0.2% HCOOH in H2O

Mobile Phase B:

0.2% HCOOH in ACN

Flow Rate:

0.556 mL/min

Isocratic Mobile Phase Composition:

8% A; 92% B

Injection Volume:

0.4 μL

Sample Concentration:

83 μg/mL

Sample Diluent:

0.02 N NaOH in 60:40 ACN:H2O

Temperature:

65 °C

Detection:

UV @ 254 nm

Sampling Rate:

20 pts/sec

Time Constant:

0.1

Instrument:

Waters ACQUITY UPLC with ACQUITY TUV

Results and Discussion

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WA60137, August 2009

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