Analysis of Food Sugars in Molasses Using ACQUITY UPLC BEH Amide Columns

Column:

ACQUITY UPLC BEH Amide 2.1 x 150 mm, 1.7 μm

Part Number:

186004802

Mobile Phase A:

80/20 MeCN/H₂O with 0.2% triethylamine [TEA]

Mobile Phase B:

30/70 MeCN/H₂O with 0.2% triethylamine [TEA]

Flow Rate:

0.29 mL/min

Flow Profile:

90% A/10% B (75% MeCN with 0.2% TEA)

Injection Volume:

2.0 μL (PLNO)

Sample Concentration:

Standards at 1 mg/mL each, molasses at 5 mg/mL

Sample Diluent:

50/50 MeCN/H₂O

Column Temperature:

35 °C

Strong Needle Wash:

20/80 MeCN/H₂O (800 μL)

Weak Needle Wash:

75/25 MeCN/H₂O (500 μL)

Seal Wash:

50/50 MeCN/H₂O

Instrument:

Waters ACQUITY UPLC with ELSD

Gain:

200

Pressure:

40 psi

Drift Tube Temperature:

40 °C

Nebulizer:

Cooling

Data Rate:

10 pps

Filter Time Constant:

Normal