• Application Note

Analysis of Analgesics Using Atlantis T3

Analysis of Analgesics Using Atlantis T3
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of analgesics.

Introduction

Compounds analysed in this application brief :

  1. Acetaminophen
  2. Caffeine
  3. 2-Acetamidophenol
  4. Acetanilide
  5. Acetylsalicyic acid
  6. Phenacetin

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 150 mm, 5 μm

Part Number:

186003747

Mobile Phase A:

H2O

Mobile Phase B:

ACN

Mobile Phase C:

1% HCOOH in H2O, pH 2.3

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Sample:

Acetaminophen (20 μg/mL), Caffeine (20 μg/mL), 2-Acetamidophenol (50 μg/mL), Acetanilide (20 μg/mL), Acetylsalicyic acid (50 μg/mL), Phenacetin (20 μg/mL) in H2O

Column Temperature:

30 ˚C

Sample Temperature:

15 ˚C

Detection:

UV @ 260 nm

Sampling Rate:

5 point/sec

Filter Response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Gradient

Time (min)

Profile

%A

%B

%C

0.00

75

15

10

10.00

30

60

10

11.00

75

15

10

15.00

75

15

10

Results and Discussion

analgesics
1. Acetaminophen, 2. Caffeine, 3. 2-Acetamidophenol, 4. Acetanilide, 5. Acetylsalicyic acid, 6. Phenacetin

Featured Products

WA60217, January 2009

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