• Application Note

ACQUITY UPLC Analysis of Organic Acids

ACQUITY UPLC Analysis of Organic Acids

  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the UPLC analysis of organic acids.

Introduction

Structures

Structures

Experimental

UPLC Conditions

Column:

ACQUITY BEH Amide, 2.1 x 100 mm, 1.7 μm

Part Number:

186004801

Mobile phase A:

50/50 MeCN/H2O with 10 mM CH3COONH4, pH 9.0

Mobile phase B:

95/5 MeCN/H2O with 10 mM CH3COONH4, pH 9.0

Gradient Flow Rate:

0.6 mL/min

Injection Volume:

5.0 μL

Column Temp:

50 °C

Sample Temp:

5 °C

Strong/Weak needle wash:

95/5 MeCN/H2O

Seal wash:

10/90 MeOH/H2O

Instrument:

ACQUITY UPLC and TQD

Gradient:

Time (min)

%A

%B

Initial

0.1

99.9

0.4

0.1

99.9

0.5

40.0

60.0

2.0

70.0

30.0

2.01

0.1

99.9

5.0

0.1

99.9

MS Conditions

Instrument:

ACQUITY TQD

Ionization Mode:

ES-

Capillary Voltage:

4.0 kV

Cone Voltage:

-25 V

Collision Energy:

10 eV

Extractor:

3 V

RF Lens:

0.1 V

Source Temp:

130 °C

Desolvation Temp:

350 °C

Desolvation Gas:

650 L/hr

Cone Gas:

0 L/hr

Collision Gas:

0.1 mL/min

MRM condition:

Pyruvic acid: 86.92 > 42.9

Lactic acid: 88.92 > 42.9

Succinic acid: 116.93 > 72.9

Maleic and Fumaric acid: 114.88 > 70.9

Results and Discussion

Compounds

WA60096, June 2009

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