• Application Note

Determination of Polycyclic Aromatic Hydrocarbons in Drinking Water by Liquid-Solid Extraction and High Performance Liquid Chromatography with Ultraviolet Detection

Determination of Polycyclic Aromatic Hydrocarbons in Drinking Water by Liquid-Solid Extraction and High Performance Liquid Chromatography with Ultraviolet Detection

  • Waters Corporation
Filling glass of water from kitchen faucet

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

In this application brief, Polycyclic aromatic hydrocarbons (PAHs) are determined in drinking water by Liquid- Solid extraction and High Performance Liquid Chromatography with Ultraviolet detection.

Introduction

Polycyclic aromatic hydrocarbons (PAHs) are one of the most wide-spread organic pollutants. PAHs are made up of fused aromatic rings and are formed during the combustion of carbon based fuels (wood, coal, diesel), as well as being present in crude oil. The United States Environmental Protection Agency (US EPA) has classified seven PAH compounds as being potentially carcinogenic including benz[a]anthra-cene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene.

Experimental

HPLC conditions

Instrument:

Waters Alliance HPLC system with PDA and fluorescence detectors

Eluent:

Water/acetonitrile (Table 1)

Column:

Waters PAH 4.6 x 250 mm (part no. 186001265) @ 30 °C

Injection:

20 μL of Supelco Standard EPA 610 (#48743) diluted 1:50 in 40:60 water/acetonitrile

Flow Rate:

1.2 mL/min

Detection:

UV @ 254 nm and fluorescence using timed programmed wavelengths

Data:

Waters Empower software

Sample preparation

Extract with C18 cartridge, elute with MeCl2

Eluent preparation

Filter and degas through a 0.45 μm filter.

A: Water

B: Acetonitrile

Eluent gradient

Time

Flow

%A

%B

Curve

Initial

1.2

40

60

-

12

1.2

0

100

9

23

1.2

40

60

11

Results and Discussion

PAH target analytes.

Standard chromatogram, UV @ 254 nm, 1-20 ppm PAH analytes. Standard chromatogram, UV @ 254 nm, 1-20 ppm PAH analytes.
Standard chromatogram, fluorescence/programmed wavelengths, 1-20 ppm PAH analytes. Standard chromatogram, fluorescence/programmed wavelengths, 1-20 ppm PAH analytes.

720002731, August 2008

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