• Application Note

Vitamins- Water-Soluble

Vitamins- Water-Soluble
  • Waters Corporation
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Vitamins and supplements in hand

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of vitamins- water-soluble.

Introduction

The compounds used in this study are – 

Compounds 

USP Tailing Factors

Niacinamide

n.c.

Calcium Pantothenate

n.c.

Pyridoxal

1.4

Pyrodoxine Hydrochloride

1.1

Pyridoxamine Dihydrochloride

1.2

Riboflavin

1.1

Thiamine Hydrochloride

1.2

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm

Part numbers:

Column - WAT046980, PIC B6 - WAT084199

Mobile phase:

20 mM PIC B6 (hexane sulfonic acid) and 0.1% phosphoric acid, pH 2.2/methanol 75:25

Flow rate:

0.7 mL/min

Injection volume:

10 μL of mixture with component 1 at 11 μg/mL, 2 at 133 μg/mL, 3 at 17 μg/mL, 4 at 6 μg/mL, 5 at 25 μg/mL, 6 at 34 μg/mL, and 7 at 67 μg/mL

Detection:

UV @ 210 nm

Results and Discussion

Featured Products

WA31763.180, June 2003

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