• Application Note

Tricyclic Antidepressants and Metabolites

Tricyclic Antidepressants and Metabolites
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of tricyclic antidepressants and metabolites.

Introduction

The compounds used in this study are – 

Compounds 

USP Tailing Factors

Nordoxepin HCL

1.5

Doxepin HCL

1.5

Nortriptyline HCL

1.3

Amtriptyline HCL

1.2

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm

Part numbers:

WAT046980

Mobile phase:

20 mM potassium phosphate, pH 7.0/methanol 29:71

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 1 to 1.16 mg/mL

Temperature:

35 °C

Detection:

UV @ 254 nm

Results and Discussion

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WA31763.174, June 2003

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