This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of morphine and its glucuronide metabolites in serum.
The compounds used in this sudy are -
            
        | 
                     Column:  | 
                     Symmetry Shield RP8, 3.9 x 150 mm, 5 μm  | 
            
| 
                     Part number:  | 
                     WAT200655  | 
            
| 
                     Mobile phase:  | 
                     20 mM potassium phosphate, pH 6.4  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     100 μL of porcine serum extract  | 
            
| 
                     Detection:  | 
                     Fluorescence; ex 280 nm, em 355 nm  | 
            
            
        
            
        
            
        WA31763.104, June 2003