• Application Note

Estrogens in River Water at 5 ng/L - Endocrine Disruptors

Estrogens in River Water at 5 ng/L - Endocrine Disruptors
  • Waters Corporation
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Water sample from the river

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights on analysis of Estrogens in river water.

Introduction

The compounds analyzed in this application brief are Diethylstilbestrol, Estrone, Ethynylestradiol, Estradiol, Bisphenol A.

17β-Estradiol

Experimental

LC Conditions

Column:

XTerra MS C18, 2.1 x 100 mm, 3.5 μm

Part Number:

186000404

Mobile phase A:

NH4OH in water, pH 10.5

Mobile Phase B:

Acetonitrile

Injection volume:

20 μL

Flow rate:

200 μL/min, plumbed directily to detector

Instrument:

Waters Alliance Separations Module

Gradient

Gradient

MS Conditions

Instrument:

Waters/Micromass Platform LC

Interface:

Negative Electrospray (ESI-)

Multiple Selected-Ion Recording (SIR)

Core voltage 27 V
OASIS SPE Method for Endocrine Disruptors

Results and Discussion

5 ng/L Spike level
5 ng/L Spike level
Results

Featured Products

WA31764.80, June 2003

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