• Application Note

Codeine and its Glucuronide Metabolite in Human Urine by LC/UV

Codeine and its Glucuronide Metabolite in Human Urine by LC/UV
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights on the analysis of codeine and its glucuronide metabolite in human urine.

Introduction

The compounds analyzed in this study are:

  1. Ranitidine (I.S.) 
  2. Codeine-6-Glucuronide (C-6-G) 
  3. Codeine
Compounds

Experimental

HPLC Conditions

Column:

Symmetry C18, 2.1 x 150 mm, 3.5 μm

Guard column:

Symmetry C18, 2.1 x 10 mm, 3.5 μm

Part numbers:

Column - 186000174, Guard - 186000169

Mobile phase:

Methanol/Acetonitrile/0.05% TFA in water 5:5:90

Flow rate:

0.3 mL/min

Injection volume:

80 μL urine extract

Temperature:

30° C

Detection:

UV @ 220 nm (0.04 AUFS)

Results and Discussion

Clearance Study from Human Urine

Clearance Study from Human Urine
Results

Test subject took a therapeutic 60 mg oral dose of Codeine and subsequently had urine monitored over a 21 hour timeframe. Peaks of interest were confirmed by LC-MS, at the 10.3 hour timepoint, to be Codeine-6-Glucuronide and Codeine

CHROMATOGRAM OF URINE EXTRACTS: A) BLANK B) SPIKED SAMPLE

Featured Products

WA31764.63, June 2003

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