This is an Application Brief and does not contain a detailed Experimental section.
This application brief describes that the Oasis 2x4 Method is a simple, logical approach to the selection of a mixed-mode SPE sorbent and protocol. Two protocols and four sorbents provide the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis.
The Oasis methodology provides a high degree of selectivity for the extraction of analytes from complex biological matrices. By selecting the appropriate Oasis sorbent and protocol, the bioanalytical scientist is able to develop sensitive, reproducible,and robust methods.
Follow the simple steps outlined in this flow chart to achieve high recoveries and the cleanest extracts:
To demonstrate the logic, simplicity, and effectiveness of the Oasis 2x4 method, five samples of rat plasma were prepared, each spiked with one of the previously characterized test analytes shown below:
Each plasma sample was diluted [1:1, v:v] and acidified with phosphoric acid [4% in water]. Respective aliquots were then processed on Oasis μElution sorbent selection plate, using the protocol and the Oasis mixed-mode sorbent designated by the Oasis 2x4 Method for the corresponding sample type. LC-MS/MS analysis was used to determine SPE recoveries. The neutral analyte was processed on all four sorbents used.
The Oasis Sorbent Selection Plates and Cartridge Kit were developed to facilitate rapid development of SPE methods for LC-MS analysis. Having all four Oasis mixed-mode sorbents (MCX, MAX, WAX and WCX) in a single plate is convenient for scouting the best ways to accomplish efficient isolation of unknown, zwitterionic compounds, or mixtures of analytes with different retention/elutionproperties.
When isolating a range of acidic, basic, and neutral compounds, a generic method using Oasis HLB (reveresed-phase only sorbent) is recommended to remove unretained matrix constituents [e.g., salts, sugars, polar lipids, proteins) while reproducibly retaining, and subsequently eluting, a broad chromatographic polarity range of acidic, basic, and neutral analytes.
When transferring methods from C18-bonded-silica phases, consider these four unique advantages of Oasis HLB:
The Oasis 2x4 Method is a simple, logical approach to the selection of a mixed-mode SPE sorbent and protocol. Two protocols and four sorbents provide the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis. The generic HLB method provides a simple, universal solution for simultaneously extracting a broad range of compounds from biological matrices.
720003679, September 2010